EFFICIENCY OF MORINGA OLEIFERA AS A NATURAL COAGULATION AND FILTRATION AGENT FOR REMOVAL OF MINERALS FROM HARD WATER: AN IN-DEPTH ANALYSIS OF MOLECULAR MECHANISMS, ADSORPTION KINETICS, AND TECHNO-ECONOMIC FEASIBILITY
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Keywords

Moringa oleifera; water treatment; water hardness; natural coagulation; hybrid filtration; adsorption isotherms; pseudo-second-order kinetics; economic analysis; sustainable technologies.

How to Cite

Rawell, M. . (2025). EFFICIENCY OF MORINGA OLEIFERA AS A NATURAL COAGULATION AND FILTRATION AGENT FOR REMOVAL OF MINERALS FROM HARD WATER: AN IN-DEPTH ANALYSIS OF MOLECULAR MECHANISMS, ADSORPTION KINETICS, AND TECHNO-ECONOMIC FEASIBILITY. Journal of Interdisciplinary Debates, 6(04), 94-112. https://doi.org/10.51249/jid.v6i04.2695

Abstract

The increasing demand for drinking water and the environmental impacts associated with conventional chemical treatments have driven research into clean and sustainable technologies. Water hardness, caused primarily by calcium ions (Ca²+) and magnesium (Mg²+), poses a significant challenge in domestic and industrial contexts. This study evaluates, through an in-depth literature review and a detailed proposal of an experimental model, the efficiency of Moringa oleifera (MO) as a natural coagulant and filtration agent for the removal of mineral ions (Ca²+, Mg²+, Fe²+/Fe3+, Zn²+ ) from hard water. The proposed methodology involves the development of a hybrid gravitational filtration system composed of activated carbon beds with controlled particle size and the application of coagulant based on OM seeds. The analysis of previous studies indicates that the cationic proteins present in OM seeds, with molecular weights between 6.5 and 48 kDa and isoelectric point above pH 10, they act effectively through adsorption mechanisms (Langmuir and Freundlich isotherms), neutralization of charge and precipitation, promoting agglutination and subsequent removal of dissolved particles and ions. The literature reports turbidity removal rates of more than 99%, pathogenic microorganisms (> log 6 for E. coli) and metals such as iron up to 100%. Kinetic studies demonstrate that the adsorption process follows pseudo-second-order models, indicating chemisorption as the predominant mechanism. Comparative economic analysis reveals that the costs of treatment with OM can be up to 50% lower than those of aluminum sulfate in rural contexts. Based on these data, it is projected that the proposed biotechnological system will achieve mineral removal efficiencies greater than 95%, consolidating itself as a low-cost, ecologically viable and high-performance alternative for hard water treatment, applicable at various scales and socioeconomic contexts.

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References

MUYIBI, S. A.; EVISON, L. M. Moringa oleifera seeds for softening hardwater. Water Research, Oxford, v. 29, n. 4, p. 1099-1104, 1995. Available at: https://doi.org/10.1016/0043-1354(94)00250-B. Accessed on: 11 nov. 2025.

FAHMI, M. R.; NAJIB, N.; PING, P. C. Mechanism of turbidity and hardness removal in hard water sources by using Moringa oleifera. Journal of Applied Sciences, v. 11, n. 16, p. 2947-2953, 2011.

DE GISI, S. et al. Characteristics and adsorption capacities of low-cost sorbents for wastewater treatment: A review. Sustainable Materials and Technologies, v. 9, p. 10-40, 2016.

BRATBY, J. Coagulation and flocculation in water and wastewater treatment. 3. ed. London: IWA Publishing, 2016.

HARLAND, C. E. Ion exchange: Theory and practice. 2nd ed. Cambridge: Royal Society of Chemistry, 1994.

BAKER, R. W. Membrane technology and applications. Hoboken: John Wiley & Sons, 2012.

NORDMARK, B. A.; PRZYBYCIEN, T. M.; TILTON, R. D. Comparative coagulation performance study of Moringa oleifera cationic protein fractions with varying water hardness. Journal of Environmental Chemical Engineering, v. 4, n. 4, p. 4690-4698, 2016. Available at: https://doi.org/10.1016/j.jece.2016.10.029. Accessed on: 11 nov. 2025.

XIONG, B. et al. Moringa oleifera f-sand filters for sustainable water purification. ACS Sustainable Chemistry & Engineering, v. 6, n. 1, p. 38-42, 2018. Available at: https://doi.org/10.1021/acssuschemeng.7b03188. Accessed on: 11 nov. 2025.

DUAN, J.; GREGORY, J. Coagulation by hydrolysing metal salts. Advances in Colloid and Interface Science, v. 100, p. 475-502, 2003.

CHOY, S. Y. et al. Utilization of plant-based natural coagulants as future alternatives towards sustainable water clarification. Journal of Environmental Sciences, v. 26, n. 11, p. 2178-2189, 2014.

YIN, C. Y. Emerging usage of plant-based coagulants for water and wastewater treatment. Process Biochemistry, v. 45, n. 9, p. 1437-1444, 2010.

GOPALAKRISHNAN, L.; DORIYA, K.; KUMAR, D. S. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, v. 5, n. 2, p. 49-56, 2016.

NDABIGENGESERE, A.; NARASIAH, K. S. Use of Moringa oleifera seeds as a primary coagulant in wastewater treatment. Environmental Technology, v. 19, n. 8, p. 789-800, 1998. Available at: https://doi.org/10.1080/09593331908616735. Accessed on: 11 nov. 2025.

TAIWO, A. S.; ADEKUNLE, A. A.; ODENIYI, O. A. Efficacy of a natural coagulant protein from Moringa oleifera (Lam) seeds in treatment of surface and underground water. Heliyon, v. 6, n. 3, e03582, 2020. Available at: https://doi.org/10.1016/j.heliyon.2020.e03582. Accessed on: 11 nov. 2025.

GASSENSCHMIDT, U. et al. Isolation and characterization of a flocculating protein from Moringa oleifera Lam. Biochimica et Biophysica Acta (BBA)-General Subjects, v. 1243, n. 3, p. 477-481, 1995.

ARREOLA, M. M. S.; CANEPA, J. R. L.; BARAJAS, J. R. H. Molecular characterization of crude seed extracts from Moringa oleifera. Interciencia, v. 41, n. 12, p. 814-820, 2016.

PATIL, D. et al. Characterization of Moringa oleifera leaf and seed protein extracts and their emulsifying properties. Food Bioscience, v. 47, 101650, 2022.

JAIN, A. et al. Preparation, characterization and functional properties of Moringa oleifera seed protein isolate. Journal of Food Science and Technology, v. 56, n. 4, p. 2093-2104, 2019.

HUNTER, R. J. Foundations of colloid science. 2. ed. Oxford: Oxford University Press, 2001.

GREGORY, J.; DUAN, J. Hydrolyzing metal salts as coagulants. Pure and Applied Chemistry, v. 73, n. 12, p. 2017-2026, 2001.

VARSANI, V. et al. Development of bio-based material from the Moringa oleifera and its bio-coagulation kinetic modeling—A sustainable approach to treat the wastewater. Heliyon, v. 8, n. 8, e10447, 2022. Available at: https://doi.org/10.1016/j.heliyon.2022.e10447. Accessed on: 11 nov. 2025.

RASHEED, F. A.; HADI, A. S.; HAMZA, A. A. The potential of Moringa oleifera seed in water coagulation-flocculation technique to reduce water turbidity. Water, Air, & Soil Pollution, v. 234, n. 4, 238, 2023. Available at: https://doi.org/10.1007/s11270-023-06238-3. Accessed on: 11 nov. 2025.

ÇELEKLİ, A.; AL-NUAIMI, A. I.; BOZKURT, H. Adsorption kinetic and isotherms of Reactive Red 120 on Moringa oleifera seed as an eco-friendly process. Journal of Molecular Structure, v. 1195, p. 168-178, 2019. Available at: https://doi.org/10.1016/j.molstruc.2019.05.106. Accessed on: 11 nov. 2025.

MATOUQ, M. et al. The adsorption kinetics and modeling for heavy metals removal from wastewater by Moringa pods. Journal of Environmental Chemical Engineering, v. 3, n. 2, p. 775-784, 2015.

REDDY, D. H. K. et al. Biosorption of $text{Pb}^{2+}$ from aqueous solutions by Moringa oleifera bark: Equilibrium and kinetic studies. Journal of Hazardous Materials, v. 174, n. 1-3, p. 831-838, 2010.

DESTA, W. M.; BOTE, M. E. Wastewater treatment using a natural coagulant (Moringa oleifera seeds): optimization through response surface methodology. Heliyon, v. 7, n. 11, e08451, 2021. Available at: https://doi.org/10.1016/j.heliyon.2021.e08451. Accessed on: 11 nov. 2025.

KENEA, D.; TSEGAYE, A.; LETA, S. Investigation on surface water treatment using blended Moringa oleifera and Aloe vera as natural coagulants. South African Journal of Chemical Engineering, v. 44, p. 213-221, 2023. Available at: https://doi.org/10.1016/j.sajce.2023.03.003. Accessed on: 11 nov. 2025.

ARNOLDSSON, E. et al. Assessment of drinking water treatment using Moringa oleifera natural coagulant. Vatten-Journal of Water Management and Research, v. 64, n. 2, p. 137-150, 2008.

OGUNSHINA, M. S. et al. Moringa oleifera coagulation characteristics in water treatment. Journal of Environmental Science and Public Health, v. 3, n. 2, p. 233-237, 2019.

MARZOUGUI, N. et al. Efficiency of different Moringa oleifera (Lam.) varieties as natural coagulants for urban wastewater treatment. Sustainability, v. 13, n. 23, 13500, 2021. Available at: https://doi.org/10.3390/su132313500. Accessed on: 11 nov. 2025.

ALI, E. N.; MUYIBI, S. A.; SALLEH, H. M. Removal of heavy metals from water and wastewater using Moringa oleifera. In: Moringa oleifera: A plant of multiple uses. London: IntechOpen, 2020. p. 1-15.

DANDESA, B.; GEBREYOHANNES, A. Y.; SIME, T. Water purification improvement using moringa oleifera seed extract pastes for coagulation follow scoria filtration. Heliyon, v. 9, n. 6, e17420, 2023. Available at: https://doi.org/10.1016/j.heliyon.2023.e17420. Accessed on: 11 nov. 2025.

RICHARDSON, S. D.; POSTIGO, C. Drinking water disinfection by-products. In: Emerging organic contaminants and human health. Berlin: Springer, 2012. p. 93-137.

CALDEIRA, N. C. A. Evaluation of Moringa oleifera Lam for removal of hardness from waters. 2012. Dissertation (Master’s Degree in Sanitation, Environment and Water Resources) - Federal University of Minas Gerais, Belo Horizonte, 2012.

NTIBREY, R. A. K.; BAYOR, M. T.; DZOMEKU, M. Antimicrobial and coagulation potential of Moringa oleifera seed: A natural approach for water treatment. Heliyon, v. 6, n. 8, e04658, 2020. Available at: https://doi.org/10.1016/j.heliyon.2020.e04658. Accessed on: 11 nov. 2025.

EILERT, U.; WOLTERS, B.; NAHRSTEDT, A. The antibiotic principle of Moringa oleifera and Moringa stenopetala. Planta Medica, v. 42, n. 05, p. 55-61, 1981.

SUAREZ, M. et al. Structure-function characterization and optimization of a plant-derived antibacterial peptide. Antimicrobial Agents and Chemotherapy, v. 49, n. 9, p. 3847-3857, 2005.

SANTOS, T. R. T. et al. Study of trihalomethane minimization by combined coagulation/flocculation process with Moringa oleifera coagulant and activated carbon filter. Blucher Chemical Engineering Proceedings, v. 1, n. 2, p. 9447-9454, 2015.

AHMED, M. Design, construction and evaluation of charcoal, activated carbon, moringa seed and sand water filtration systems. 2019. Thesis (Doctorate) - African University of Science and Technology, Abuja, 2019.

GANDIWA, B. I. et al. Optimisation of using a blend of plant based natural and synthetic coagulants for water treatment:(Moringa Oleifera-Cactus Opuntia-alum blend). South African Journal of Chemical Engineering, v. 33, p. 138-146, 2020. Available at: https://doi.org/10.1016/j.sajce.2020.07.005. Accessed on: 11 nov. 2025.

PRITCHARD, M. et al. Potential of using plant extracts for purification of shallow well water in Malawi. Physics and Chemistry of the Earth, Parts A/B/C, v. 35, n. 13-14, p. 791-797, 2010.

ZEA COBOS, A. G.; RAMÍREZ ARCILA, H.; RODRÍGUEZ, J. P. Use of Moringa oleifera as a natural coagulant in the treatment of water and wastewater: A review. Water, v. 16, n. 16, 2315, 2024. Available at: https://doi.org/10.3390/w16162315. Accessed on: 11 nov. 2025.

SALAZAR GÁMEZ, L. L. et al. Comparative study between M. oleifera and aluminum sulfate for water treatment: case study Colombia. Environmental Monitoring and Assessment, v. 187, n. 9, p. 1-12, 2015.

ROCHA, V. V. F. et al. Clarification of high-turbidity waters: a comparison of Moringa oleifera and virgin and recovered aluminum sulfate-based coagulants. Environment, Development and Sustainability, v. 22, n. 8, p. 7739-7753, 2020.

BELTRAN-HEREDIA, J.; SANCHEZ-MARTIN, J.; DELGADO-REGALADO, A. Removal of carmine indigo dye with Moringa oleifera seed extract. Industrial & Engineering Chemistry Research, v. 48, n. 14, p. 6512-6520, 2009.

BERGER, M. R. et al. Toxicological assessment of seeds from Moringa oleifera and Moringa stenopetala, two efficient primary coagulants for domestic water treatment of tropical raw waters. East African Medical Journal, v. 61, n. 9, p. 712-716, 1984.

GRABOW, W. O. K. et al. Toxicity and mutagenicity evaluation of water coagulated with Moringa oleifera seed preparations using fish, protozoan, bacterial, coliphage, enzyme and Ames Salmonella assays. Water SA, v. 11, n. 1, p. 9-14, 1985.

YUSOFF, M. A. M. et al. Analysis of water treatment by Moringa oleifera bioflocculant: Economic feasibility. Polish Journal of Environmental Studies, v. 26, n. 5, p. 2337-2344, 2017.

DELFÍN-PORTELA, E. et al. Estimation of processing times and economic feasibility of Moringa oleifera production using simulation modeling. Applied Sciences, v. 14, n. 16, 7225, 2024. Available at: https://doi.org/10.3390/app14167225. Accessed on: 11 nov. 2025.

KILINGO, F. M.; NJAU, K. N.; MACHUNDA, R. L. Study of domestic wastewater treatment using Moringa oleifera as a primary coagulant and sand filtration. Scientific African, v. 15, e01060, 2022.

GUPTA, R. R. Use of natural coagulants in water treatment: A comparative study of Moringa oleifera and alum. Authorea Preprints, 2025.

MUYIBI, S. A. et al. Effects of oil extraction from Moringa oleifera seeds on coagulation of turbid water. International Journal of Environmental Studies, v. 60, n. 4, p. 383-395, 2003.

SUTHERLAND, J. P. et al. Moringa oleifera at pilot/full scale. In: WEDC CONFERENCE, 19., 1993, Accra, Ghana. Proceedings... Accra, Ghana, 1993.

FOLKARD, G.; SUTHERLAND, J. Development of a naturally derived coagulant for water and wastewater treatment. Water Supply, v. 2, n. 5-6, p. 89-94, 2002.

OKUDA, T. et al. Coagulation mechanism of salt solution-extracted active component in Moringa oleifera seeds. Water Research, v. 35, n. 3, p. 830-834, 2001.

BRADFORD, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, v. 72, n. 1-2, p. 248-254, 1976.

CRITTENDEN, J. C. et al. MWH’s water treatment: Principles and design. Hoboken: John Wiley & Sons, 2012.

MARSH, H.; RODRÍGUEZ-REINOSO, F. Activated carbon. Amsterdam: Elsevier, 2006.

BANSAL, R. C.; GOYAL, M. Activated carbon adsorption. Boca Raton: CRC Press, 2005.

STUMM, W.; MORGAN, J. J. Aquatic chemistry: Chemical equilibria and rates in natural waters. Hoboken: John Wiley & Sons, 2012.

HO, Y. S.; MCKAY, G. Pseudo-second order model for sorption processes. Process Biochemistry, v. 34, n. 5, p. 451-465, 1999.

BRAZIL. Ministry of Health. Ordinance GM/MS No. 888, of May 4, 2021. Amends Annex XX of Consolidation Ordinance GM/MS No. 5, of September 28, 2017, to provide for the control and surveillance procedures of the quality of water for human consumption and its potability standard. Federal Official Gazette, Brasília, DF, May 7, 2021.

RANADE, V. V.; BHANDARI, V. M. Industrial wastewater treatment, recycling and reuse. Oxford: Butterworth-Heinemann, 2014.

GREENLEE, L. F. et al. Reverse osmosis desalination: Water sources, technology, and today’s challenges. Water Research, v. 43, n. 9, p. 2317-2348, 2009.

ANWAR, F. et al. Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research, v. 21, n. 1, p. 17-25, 2007.

PALADA, M. C.; CHANG, L. C. Suggested cultural practices for Moringa. International Cooperators’ Guide, AVRDC Publication, 03-545, 2003.

OKUDA, T. et al. Improvement of extraction method of coagulation active components from Moringa oleifera seed. Water Research, v. 33, n. 15, p. 3373-3378, 1999.

LEA, M. Bioremediation of turbid surface water using seed extract from Moringa oleifera Lam. (drumstick) tree. Current Protocols in Microbiology, v. 16, n. 1, 1G-2, 2010.