234
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
COLLAGEN BIOSTIMULATORS IN FACIAL REJUVENATION
TREATMENT
Rosania Henriques1
Nicola Felipe Lopez Bempensante2
Alessandra Marostica De Freitas3
Adriana Takeda4
Juliana Brasil Arioli Nieto5
Elka Bonetti6
Roberto Teruo Suguihara7
Abstract: This study aims to investigate the efcacy and safety of collagen bio-stimulators in facial
aesthetic procedures, focusing on promoting skin elasticity and rmness. The justication for this
research is based on the increasing demand for minimally invasive treatments that provide satisfactory
results in facial rejuvenation. The adopted methodology consists of a literature review, analyzing
scientic articles, systematic reviews, and other relevant materials published in the last ten years. The
discussion highlights the effectiveness of bio-stimulators, such as calcium hydroxyapatite and poly-
L-lactic acid, compared to surgical treatments, as well as the importance of personalizing procedures
and combining them with other aesthetic techniques. The conclusion reafrms that the research
question was positively answered and that the specic objectives were achieved, recommending
further research to explore new formulations and their implications.
1 Postgraduate Studies at Innovare College. https://orcid.org/0009-0009-9564-2172
2 Postgraduate Studies at Innovare College.. https://orcid.org/0009-0000-1568-8379
3 Anhanguera College. https://orcid.org/0009-0003-4681-3904
4 Postgraduate Studies at Innovare College. https://orcid.org/0009-0002-8660-3787
5 Postgraduate Studies at Innovare College. https://orcid.org/0000-0001-9237-2378
6 Postgraduate Studies at Innovare College. https://orcid.org/0000-0001-8475-5539
7 Italian-Brazilian Catholic Center/Anatomy. https://orcid.org/0000-0002-2302-2427
235
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
Keywords: bio-stimulators, rejuvenation, aesthetics.
INTRODUCTION
The use of collagen biostimulators in facial rejuvenation treatment has become an increasingly
common practice in aesthetic dermatology, due to their ability to stimulate collagen production and
improve the appearance of the skin.
Biostimulators, such as calcium hydroxyapatite (CaHA) and poly-L-lactic acid (PLLA),
act in the deeper layers of the skin, promoting a controlled inammatory response that results in
neocollagenesis, that is, the formation of new collagen bers (Luiz, 2023; Neca et al., 2022; Garcia,
2024). This approach not only improves skin elasticity and rmness, but also provides a facial
volumizing effect, which is essential for restoring youthfulness lost with aging (Rodrigues et al.,
2022; Matos, 2023).
Calcium hydroxyapatite, in particular, is one of the most studied and used biostimulators in
clinical practice. This material not only acts as a ller but also stimulates collagen production over
time, resulting in sustainable improvements in skin texture and appearance (Troczinski, 2024; Silva,
2024).
Studies show that the application of CaHA can lead to visible results that are maintained
for several months, making it an attractive option for patients seeking facial rejuvenation without the
need for invasive surgical interventions (Neca et al., 2022; Beserra et al., 2023).
The problem question that guides this research is: what are the effects of collagen biostimulators
on facial rejuvenation and what is the available evidence on their efcacy and safety?
The rationale for this study lies in the fact that skin aging is a natural process, which results
in loss of collagen, elasticity, and hydration, leading to the appearance of wrinkles and sagging. In
this context, collagen biostimulators emerge as a promising alternative to promote skin regeneration
and improve facial appearance. A review of the literature on this topic is essential to better understand
236
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
the benets, mechanisms of action, and potential risks of these treatments, contributing to a more
informed and safe clinical practice. In addition, the growing demand for aesthetic procedures that use
products with a lower risk of adverse effects justies the need to investigate effective and safe options,
such as biostimulators.
The general objective of this article is to investigate the efcacy of collagen biostimulators
in the treatment of facial rejuvenation, analyzing their properties, mechanisms of action, and clinical
results. To achieve this objective, three specic objectives were established: the rst is to review the
existing literature on the different types of collagen biostimulators used in facial aesthetics, their
formulations and indications.
The second objective is to evaluate the effects of collagen biostimulators on skin quality
parameters, such as elasticity, hydration and wrinkle reduction. Finally, the third objective aims to
analyze the safety and side effects associated with the use of collagen biostimulators in facial aesthetic
procedures.
METHODOLOGY
The methodology used for this research will consist of a literature review with a bibliographic
focus, with the objective of compiling and analyzing existing studies on the application of collagen
biostimulators in facial aesthetic procedures. The review will be carried out in several stages, starting
with the denition of inclusion and exclusion criteria for the selection of articles to be considered.
Scientic articles, systematic reviews, theses, dissertations, and books published in peer-reviewed
journals in the areas of dermatology, aesthetic medicine, and health sciences will be included.
The research will be carried out in academic databases, such as PubMed, Scopus, Google
Scholar and Web of Science, using specic keywords such as “collagen biostimulators”, “calcium
hydroxyapatite”, “poly-L-lactic acid”, “neocollagenesis” and “aesthetic treatments”. The search will
cover publications from the last ten years to ensure the relevance and timeliness of the information.
237
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
The selected articles will undergo a critical analysis, considering the methods used, the
results obtained and the conclusions presented by the authors. This analysis will seek to identify
patterns and divergences in the evidence on the efcacy, safety, and possible side effects related to the
use of collagen biostimulators. Additionally, the combinations of biostimulators with other aesthetic
techniques, such as microneedling and radiofrequency, will be investigated to evaluate the synergy
between the treatments and their impacts on aesthetic results.
The data collected will be organized into tables and summaries, allowing for a clear synthesis
of the information and facilitating comparison between the different studies. The literature review will
result in the elaboration of a text that addresses the evolution of the use of collagen biostimulators, their
indications and contraindications, in addition to discussing the efcacy and safety of these treatments,
as well as the emotional and psychological implications they may have on the self-esteem of patients.
This methodological approach aims to contribute to a deeper understanding of collagen
biostimulators, providing a solid foundation for future research and clinical practices in the aesthetic
area. Through the literature review, it is expected to identify gaps in current knowledge and suggest
areas that need further investigations, thus promoting an advance in aesthetic practices and patient
satisfaction.
LITERATURE REVIEW
Different types of collagen biostimulators used in facial aesthetics, their formulations and
indications
Collagen biostimulators are consolidating themselves as an essential tool in facial aesthetics,
presenting a minimally invasive alternative for skin rejuvenation. These products act on the deep
layers of the dermis, stimulating collagen production and thus improving the elasticity and rmness
of the skin. Among the most commonly used biostimulators, calcium hydroxyapatite (CaHA) and
238
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
poly-L-lactic acid (PLLA) stand out, each with specic characteristics in terms of formulation and
clinical indications.
Calcium hydroxyapatite, widely used, is a material that combines biocompatibility and
biodegradability, working both as a ller and as a biostimulator. Its application triggers a controlled
inammatory response that induces neocollagenesis, or the formation of new collagen bers. Research
indicates that CaHA is effective in restoring facial volumes and improving skin texture, with results
that can last for up to 18 months after application (Luiz, 2023; Neca et al., 2022; Troczinski, 2024).
In addition, calcium hydroxyapatite is considered safe, with a low incidence of adverse effects, which
makes it a popular choice among aesthetic professionals (Miranda & Lopes, 2023; Araújo et al., 2022).
On the other hand, poly-L-lactic acid is a biostimulator that, when injected, also promotes
collagen production, although its mechanism of action is somewhat different. This compound is
recognized for its ability to induce collagen formation over time, with results becoming more evident
after a few weeks and can last up to two years (Farina & Mota, 2023; Nogueira & Silva, 2022). PLLA
is often used in areas that require a gradual increase in volume, such as the cheeks and jawline, and
is especially indicated for patients looking for a subtle and natural effect (Jacintho, 2023; Zaragoza,
2023).
In addition to calcium hydroxyapatite and poly-L-lactic acid, other biostimulators have gained
relevance in aesthetic practice. Hyaluronic acid, traditionally used as a ller, also has biostimulatory
properties when applied in specic concentrations and in combination with techniques such as
microneedling (Machado, 2023; Pires & Ribeiro, 2021). The use of hyaluronic acid can not only ll
in furrows and wrinkles, but also improve skin hydration and elasticity, contributing to a younger and
healthier appearance (Rodrigues et al., 2022; Lima & Soares, 2020).
Collagen biostimulators are often integrated into combined protocols, where different
substances are used together to maximize aesthetic results. For example, the association of calcium
hydroxyapatite with microneedling has shown promising results in the treatment of sagging skin, since
microneedling enhances the absorption of biostimulators and further stimulates collagen production
239
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
(Farina & Mota, 2023; Sinigaglia & Führ, 2019). This integrated approach not only improves skin
texture but also promotes a lifting effect, which is highly desired by patients looking for facial
rejuvenation.
The selection of the appropriate biostimulator must consider several factors, such as the
patient’s skin condition, their expectations, and the place of application. The experience of the
professional responsible for the procedure is essential to ensure satisfactory results and minimize the
risk of complications (Beserra et al., 2023; Veloso, 2023). In addition, prior assessment of the patient’s
health and discussion of possible side effects are essential steps for safe and effective treatment (Matos,
2023; Seabra & Silva, 2022).
It is important to note that collagen biostimulators are not exempt from contraindications.
Patients with autoimmune diseases, active infections in the area of application or allergies to product
components should be carefully evaluated before any procedure. Personalization of treatment, taking
into account the individual characteristics of each patient, is crucial for the success of the aesthetic
intervention (Araújo et al., 2022).
Poly-L-lactic acid, as well as calcium hydroxyapatite, stands out as an effective biostimulator.
When injected, it activates broblasts, cells responsible for collagen production, promoting a thickening
of the skin that becomes visible after a few weeks (Farina & Mota, 2023; Miyashiro et al., 2022).
The combination of both biostimulators can enhance the results, offering a synergistic effect
that improves skin quality in a comprehensive way (Farina et al., 2022; Araújo et al., 2022). The use
of minimally invasive techniques, such as the injection of biostimulators, is revolutionizing the eld
of aesthetics, allowing patients to obtain satisfactory results with a reduced recovery time (Matos,
2023; Silva et al., 2022).
Collagen biostimulators are considered safe, with a minimal side effect prole, which makes
them a popular choice among healthcare professionals and patients (Lima & Soares, 2020; Araújo
et al., 2022). The biocompatibility and biodegradability of these materials are characteristics that
favor their acceptance in the aesthetic market, allowing treatments to be performed with condence
240
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
(Veloso, 2023; Beserra et al., 2023). Personalization of treatments, taking into account the specic
needs of each patient, is essential to maximize results and minimize risks (Pires & Ribeiro, 2021;
Araújo et al., 2022).
The efcacy of collagen biostimulators in facial rejuvenation is supported by a wide scientic
literature. Studies show that the application of biostimulators not only improves the appearance
of the skin, but also contributes to patients’ self-esteem, an aspect often neglected in discussions
about aesthetic treatments (Rodrigues et al., 2022; Beserra et al., 2023). Facial harmonization, which
includes the use of biostimulators, represents a holistic approach that aims to restore individuals’
natural beauty and condence, reecting the importance of aesthetics in mental and emotional health
(Pires & Ribeiro, 2021; Silva et al., 2022).
In addition to the aesthetic benets, the use of collagen biostimulators can be analyzed from
the perspective of regenerative medicine. The ability of these materials to promote skin regeneration
and the formation of new collagen aligns with current trends that seek not only to improve appearance
but also to restore skin health at the cellular level (Troczinski, 2024; Veloso, 2023; Papaiordanou et
al., 2022)
This opens up new possibilities for the use of biostimulators in other areas of aesthetic
medicine, such as the treatment of scars and sagging (Farina & Mota, 2023).
Continued research on collagen biostimulators is vital to better understand their applications
and optimize treatment protocols. Current literature suggests that, although the results are generally
positive, the experience of the practitioner and the choice of the appropriate product are crucial for
the success of the treatment (Beserra et al., 2023; Araújo et al., 2022). The training and updating of
health professionals who perform these procedures are essential to ensure the safety and efcacy of
treatments (Jacintho, 2023; Matos, 2023).
The evolution of techniques and products in facial aesthetics, particularly in relation to
collagen biostimulators, demonstrates a growing commitment to the safety and efcacy of treatments.
With new studies conducted, it is essential for healthcare professionals to stay up-to-date on best
241
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
practices and innovations in the eld. This update not only improves patient outcomes but also
strengthens public condence in cosmetic procedures.
The scientic literature suggests that combining biostimulators with other aesthetic
approaches, such as lasers or topical treatments, can lead to signicant improvements in skin quality
and patient satisfaction. For example, the application of biostimulators after laser treatments can
enhance the regenerative effects on the skin, favoring a faster and more effective recovery (Rodrigues
et al., 2022; Araújo et al., 2022).
Another important factor is patient education. Informing patients about what to expect from
collagen biostimulator treatments is key to setting realistic expectations and ensuring satisfaction
with the results. It is crucial to discuss with patients the required number of sessions, the duration of
effects, and post-procedure care as part of the initial consultation (Matos, 2023; Veloso, 2023). This
approach helps reduce anxiety and promotes better adherence to the professional’s recommendations.
Personalization of treatment is also an essential aspect. Each patient has unique characteristics
that inuence the choice of biostimulator and the technique to be used. Considering factors such
as age, skin type, severity of sagging, and patient expectations is essential to achieve satisfactory
results (Beserra et al., 2023; Farina et al., 2022). Individualized treatment protocols can maximize the
effectiveness and safety of procedures.
Ethics in aesthetic practice must be prioritized. Professionals have the responsibility to ensure
that interventions are carried out in accordance with ethical and legal guidelines, always prioritizing
the patient’s well-being. Transparency in discussions about the expected outcomes and risks associated
with treatments is crucial to promote responsible practice (Seabra & Silva, 2022; Troczinski, 2024).
Additionally, research on collagen biostimulators is expected to continue to expand, exploring
their use in treatments for scars, stretch marks, and other aspects of skin health. The possibility of
using biostimulators not only for aesthetic purposes, but also to promote skin health and regeneration
in different contexts, is a promising eld that deserves attention and investigation (Papaiordanou et
al., 2022; Silva et al., 2022).
242
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
In summary, collagen biostimulators represent a revolution in facial aesthetics, offering
effective and safe solutions for skin rejuvenation. The combination of scientic research, ethical
practices, and personalization of treatment will be critical to the future of aesthetic medicine, ensuring
that patients can benet from advances in this area with condence and safety.
Collagen biostimulators are becoming a fundamental tool in aesthetic medicine, especially
in the context of facial harmonization and skin rejuvenation. Among the main biostimulators, poly-
L-lactic acid (PLLA), calcium hydroxyapatite (CaHA) and polydioxanone (PDO) stand out. Each
of these materials has distinct characteristics that inuence their application and effectiveness in
promoting neocollagenesis, that is, in stimulating collagen production by the skin.
Poly-L-lactic acid is a biodegradable polymer that, when injected into the junction between the
dermis and the subcutaneous, stimulates broblasts to produce collagen. This results in a volumizing
effect that becomes noticeable after about two months and can last up to two years (Farina & Mota,
2023). This biostimulator is widely used in treatments for sagging and loss of facial volume, and is
considered safe and effective in various aesthetic applications (Rodrigues et al., 2022; Zaragoza,
2023). PLLA’s ability to induce collagen production is one of the key factors that make it a popular
choice among aesthetic professionals (Farina et al., 2022).
On the other hand, calcium hydroxyapatite, which has a mineral composition similar to that
of human bones, is used both as a ller and as a biostimulator of collagen (Neca et al., 2022; Diaz et
al., 2022). This compound is known to offer immediate results in volumization and to stimulate the
gradual production of collagen. Studies indicate that calcium hydroxyapatite can improve skin texture
and restore lost volumes, being a safe and minimally invasive option for aesthetic procedures (Moura,
2023; Troczinski, 2024).
Its effectiveness is associated with the activation of broblasts, which promote the formation
of new collagen bers, contributing to the elasticity and rmness of the skin (Vasconcelos et al., 2022).
Although less discussed compared to PLLA and CaHA, polydioxanone also plays an
important role in collagen induction. This material is often used in support threads that, when inserted
243
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
into the skin, create a controlled inammatory response that stimulates collagen production (Dias
et al., 2022). The collagen induction technique with PDO threads has been shown to be effective in
treating sagging and improving skin texture, providing results that can last from one to two years
(Zaragoza, 2023; Dias et al., 2022).
The classication of collagen biostimulators can be made based on their permanence and
absorption by the body, categorizing them as temporary, semi-permanent, or permanent (Araújo
et al., 2022). Poly-L-lactic acid and calcium hydroxyapatite are considered semi-permanent, while
polydioxanone is seen as a temporary material due to its faster absorption (Nogueira & Silva, 2022).
This classication is crucial for aesthetic professionals, as it guides the choice of the most appropriate
biostimulator, taking into account the needs and expectations of patients.
In addition to the individual characteristics of each biostimulator, the combination of different
techniques and products has shown promise in maximizing aesthetic results. For example, therapy
that combines microneedling and biostimulators can enhance the effectiveness of the treatment,
promoting a more robust and comprehensive collagen induction (Farina & Mota, 2023; Tired, 2023).
The literature suggests that integrated approaches, which use multiple stimulation methods,
can result in signicant improvements in the appearance of the skin, addressing not only sagging but
also other aesthetic concerns, such as wrinkles and ne lines (Veloso, 2023).
The safety and efcacy of collagen biostimulators have been widely documented in clinical
studies, which show positive results in terms of patient satisfaction and minimization of side effects
(Moura, 2023; Pogere et al., 2021). Calcium hydroxyapatite, for example, is recognized for its
biocompatibility and low risk of complications, making it a frequent choice among aesthetic health
professionals (Oliveira et al., 2021; Bordalo, 2024). Similarly, poly-L-lactic acid is considered safe,
with a generally mild and temporary adverse effect prole (Farina & Mota, 2023; Zaragoza, 2023).
In addition to the evidence that proves the safety and efcacy of biostimulators, it is essential
to emphasize the importance of continuous training of health professionals involved in aesthetics.
Updating on new techniques, products, and protocols is crucial to ensure that procedures are performed
244
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
safely and to maximize aesthetic results. Professionals should be informed about the latest research
and trends, as well as improve their skills in patient assessment and treatment planning (Matos, 2023;
Jacintho, 2023).
Personalization of treatment is another vital factor that should not be disregarded. Each patient
has unique characteristics that inuence the choice of biostimulator and the technique to be used. It
is essential to assess the patient’s age, skin type, severity of sagging, and expectations to achieve
satisfactory results (Beserra et al., 2023; Farina et al., 2022). Therefore, designing an individualized
treatment plan not only increases effectiveness but also promotes greater patient satisfaction.
Ethics play a signicant role in aesthetic practice. Professionals must ensure that procedures
are performed in accordance with ethical and legal guidelines, always prioritizing the patient’s
well-being. In addition, transparency in discussions about the expected outcomes, the limitations
of treatments, and the associated risks is essential for responsible practice (Seabra & Silva, 2022;
Troczinski, 2024).
Considering the emotional and psychological impact that aesthetic treatments can have on
patients is also relevant. Improvement in the appearance of the skin often results in an increase in
self-esteem and self-condence, factors that must be taken into account when approaching aesthetic
treatment (Rodrigues et al., 2022; Beserra et al., 2023). This holistic perspective is essential for
understanding the role of collagen biostimulators in the mental and emotional health of individuals.
In addition, research on collagen biostimulators should continue to expand, exploring new
indications and therapeutic combinations. Investigating innovative uses, such as the treatment of scars
and stretch marks, can open up new opportunities for the application of these products in aesthetic
medicine (Papaiordanou et al., 2022; Silva et al., 2022).
In summary, collagen biostimulators represent a true revolution in aesthetic medicine, offering
effective and safe solutions for facial rejuvenation. The integration of scientic research, ethical
practice, personalization of treatment and continuous training of professionals will be fundamental
for the future of aesthetics. In this way, patients will be able to benet from advances in this area
245
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
safely and condently, leading to results that not only improve appearance, but also positively impact
self-esteem and quality of life.
Effects of collagen biostimulators on skin quality parameters such as elasticity, hydration, and
wrinkle reduction
Collagen biostimulators have gained notoriety in aesthetics, especially with regard to the
improvement of skin quality parameters, such as elasticity, hydration, and wrinkle reduction. These
agents work by promoting the production of collagen, a protein that is essential for maintaining skin
structure and rmness. The natural degradation of collagen with aging results in sagging, wrinkles,
and loss of elasticity, leading many individuals to seek aesthetic interventions to reverse these effects
(Machado, 2023; Santos et al., 2023; Neca et al., 2022).
Biostimulators, such as calcium hydroxyapatite (CaHA) and poly-L-lactic acid (PLLA), have
been shown to be effective in promoting neocollagenesis, which is the formation of new collagen
bers. CaHA, for example, acts as a ller that offers immediate volume, while stimulating collagen
production over time, resulting in rmer and more elastic skin (Neca et al., 2022; Troczinski, 2024;
Silva, 2024). Studies indicate that the application of these biostimulators can result in signicant
improvements in skin texture, with visible results that can last for up to two years after treatment
(Farina & Mota, 2023; Matos, 2023).
In addition to calcium hydroxyapatite, hyaluronic acid (HA) is widely used in aesthetic
procedures. HA not only improves skin hydration but also plays an important role in stimulating
broblasts, which are responsible for the production of collagen and elastin (Machado, 2023; Santos
et al., 2023). The combination of HA with other biostimulators has shown potential to maximize
aesthetic results, promoting a more comprehensive facial rejuvenation (Santos et al., 2023; Farina &
Mota, 2023).
Skin elasticity is one of the main parameters affected by aging and exposure to environmental
246
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
factors such as UV radiation. The application of collagen biostimulators can help restore this
elasticity, since collagen production is essential for the structure of the dermis (Seabra & Silva, 2022;
Silva, 2023). Research suggests that oral collagen supplementation may also contribute to improving
skin elasticity, as well as reducing the appearance of wrinkles and ne lines (Carvalho et al., 2023;
Miyashiro et al., 2022).
Skin hydration is another critical aspect that can be improved with the use of biostimulators.
Proper hydration is vital for maintaining the skin’s barrier function and preventing premature
aging. Stimulating collagen production helps to lock in moisture, resulting in smoother, healthier
skin (Papaiordanou et al., 2022; Miyashiro et al., 2022). The combination of topical and injectable
treatments can enhance these effects, offering a holistic approach to skin rejuvenation (Marinho,
2023).
In addition to the aesthetic benets, collagen biostimulators are considered safe and
minimally invasive, making them an attractive option for many patients. The biocompatibility and
biodegradability of these products favor their acceptance in the aesthetic market (Araújo et al., 2022;
Zaragoza, 2023). Studies show that adverse effects are minimal and usually limited to mild injection
site reactions, such as swelling and redness (Neca et al., 2022; Silva, 2024).
The effectiveness of collagen biostimulators is supported by a number of clinical studies
that demonstrate signicant improvements in skin quality. Research suggests that the combination of
different biostimulators can result in synergistic effects, maximizing aesthetic results and prolonging
the durability of the effects (Matos, 2023; Veloso, 2023; Nogueira & Silva, 2022). Personalization
of treatments, taking into account the individual needs of patients, is essential to optimize patient
outcomes and satisfaction (Seabra & Silva, 2022; Lima & Soares, 2020).
In summary, collagen biostimulators represent an innovative and effective approach in the
treatment of skin aging, positively impacting elasticity, hydration, and wrinkle reduction. Continuous
research and development in this area promises to expand the available options and further improve
aesthetic results, contributing to the self-esteem and well-being of individuals who seek these
247
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
treatments (Machado, 2023; Farina & Mota, 2023; Veloso, 2023).
The popularity of collagen biostimulators has driven the increase in clinics and professionals
offering these treatments, reecting a growing acceptance and demand in the aesthetic market. With
an increasing number of individuals seeking minimally invasive interventions to enhance their
appearance, the need for information and adequate training for professionals in the eld becomes
even more crucial. Continuous education not only ensures that professionals are up-to-date on best
practices and techniques, but also fosters patient condence in available treatments (Matos, 2023;
Jacintho, 2023).
The evolution of injection technologies, along with the introduction of new products in the
market, expands the possibilities of personalization of treatments. The combination of biostimulators
with different techniques, such as microneedling, can enhance the results, providing a synergistic
effect that favors collagen production and improves skin texture (Farina & Mota, 2023; Tired, 2023).
This integrated approach allows aesthetic professionals to offer more comprehensive solutions,
meeting the diverse needs of patients.
Pre-treatment evaluation is essential to determine the most appropriate protocol for each
patient. This involves a detailed analysis of the patient’s skin condition, medical history, and
expectations. A well-structured treatment plan not only increases the effectiveness of procedures but
also helps minimize potential risks and side effects (Seabra & Silva, 2022; Veloso, 2023). In addition,
clear communication about the expected outcomes and limitations of treatments is essential to ensure
patient satisfaction and establish a trusting relationship between the professional and the client.
The growing concern about the health and safety of aesthetic treatments underscores the
importance of following ethical and legal guidelines. Professionals should always prioritize the well-
being of patients, ensuring that procedures are performed in appropriate environments and under safe
conditions (Troczinski, 2024). Transparency regarding potential risks and expectations of results is
also critical to the responsible practice of aesthetic medicine.
With the advancement of research on collagen biostimulators, it is expected that new
248
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
applications and formulations will be developed. This may include investigations into the use of
biostimulators in areas beyond facial aesthetics, such as in the treatment of scars, stretch marks, and
body sagging (Papaiordanou et al., 2022; Silva et al., 2022). The future of collagen biostimulators
looks promising, with the potential to offer innovative solutions that not only enhance appearance but
also contribute to skin health at a cellular level.
In summary, collagen biostimulators have established themselves as a powerful tool
in aesthetic medicine, providing signicant benets in terms of elasticity, hydration, and wrinkle
reduction. As research advances and techniques evolve, these treatments are expected to become even
more effective and affordable, meeting the rising expectations of patients seeking facial rejuvenation
and wellness. The combination of science, technique, and ethics will be critical to continued success
in this expanding eld.
Collagen biostimulators have stood out as a promising alternative for the treatment of sagging
and loss of skin elasticity, conditions often associated with aging. The effectiveness of these agents in
promoting skin elasticity is supported by a number of clinical studies demonstrating their benecial
effects on dermal structure. For example, calcium hydroxyapatite is a widely used biostimulator,
which stimulates collagen production, resulting in signicant improvements in skin elasticity over
time (Neca et al., 2022; Seabra & Silva, 2022).
The skin aging process is multifactorial and results in decreased collagen and elastin
production, which leads to a loss of rmness and elasticity (Machado, 2022). The introduction of
biostimulators, such as poly-L-lactic acid, has shown positive results in neocollagenesis, which is
the formation of new collagen, essential for the recovery of skin elasticity (Farina & Mota, 2023;
Zaragoza, 2023). Studies indicate that the effects of these treatments can be noticed after a few
weeks, with results that can last up to two years, depending on the type of biostimulator used and the
individual characteristics of the patient (Farina & Mota, 2023).
In addition, the combination of different techniques, such as microneedling associated with
biostimulators, has been explored to enhance aesthetic results. This approach not only stimulates
249
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
collagen production but also improves skin texture, contributing to more comprehensive facial
rejuvenation (Farina & Mota, 2023). The safety and efcacy of biostimulators are often highlighted
in studies, which emphasize the minimally invasive nature of these procedures, making them an
attractive option for patients who want to improve skin elasticity without resorting to surgery (Neca
et al., 2022; Miranda & Lopes, 2023).
Biostimulators are classied into different categories, such as temporary, semi-permanent,
and permanent, based on their durability and absorption by the body (Miranda & Lopes, 2023).
Calcium hydroxyapatite, for example, is considered a semi-permanent ller that not only provides
volume, but also stimulates collagen production, offering results that can last from 18 months to ve
years (Miranda & Lopes, 2023). This characteristic is especially relevant for patients who seek long-
lasting results without the need for multiple treatment sessions.
The effectiveness of biostimulators on skin elasticity is also corroborated by studies showing
signicant improvements in skin rmness and texture after treatment (Zaragoza, 2023; Veloso, 2023).
The use of biostimulators, such as poly-L-lactic acid and polycaprolactone, has shown promising
results not only in reducing wrinkles, but also in improving skin thickness and elasticity (Veloso,
2023). These results are particularly relevant in a context where the demand for effective and safe
aesthetic solutions is constantly growing.
In addition to the aesthetic benets, the use of biostimulators also has positive implications
for the self-esteem and psychosocial well-being of patients. Improvement in the appearance of the
skin can lead to a signicant increase in condence and personal satisfaction, factors that are often
underestimated in discussions about aesthetic treatments (Machado, 2022). The search for treatments
that not only treat sagging, but also promote global skin rejuvenation, reects a change in patients’
expectations regarding aesthetic care.
In this way, biostimulators represent a class of treatments that not only address sagging
skin but also offer a holistic approach to facial rejuvenation. The combination of different techniques
and products, such as the association of biostimulators with procedures such as microneedling, can
250
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
maximize results and provide a more satisfactory experience for patients (Farina & Mota, 2023). The
future of aesthetic treatments looks promising, with the continuous research and development of new
formulations and techniques that aim to improve the efcacy and safety of biostimulators.
The current literature suggests that the choice of biostimulator should be individualized,
taking into account the specic characteristics of the patient’s skin, their expectations, and the type
of sagging to be treated (Morais, 2023). Personalization of treatment is key to ensuring optimal
outcomes and patient satisfaction. Additionally, it is crucial to educate patients about the different
types of biostimulators, their durability, and effects, so that they can make informed decisions about
their aesthetic treatments (Miranda & Lopes, 2023).
Lastly, ongoing research into the effectiveness of biostimulators on skin elasticity is vital for
advancing aesthetic practices. Future studies should focus not only on clinical efcacy, but also on
long-term safety and patient satisfaction, ensuring that the treatments offered are effective, safe, and
sustainable (Veloso, 2023). The integration of new technologies and innovative approaches in the eld
of biostimulators can result in even more remarkable results, solidifying its position as a preferred
solution for facial rejuvenation.
The demand for aesthetic treatments with collagen biostimulators is growing as more
people look for effective ways to combat the signs of aging. Awareness of the benets and safety
of these products is increasing, resulting in increased acceptance among the public. Therefore, it is
essential that aesthetic professionals strive to provide accurate and evidence-based information about
biostimulators, highlighting their properties, modes of action, and expected results.
The personalization of treatments goes beyond the simple choice of the type of biostimulator,
also encompassing the adaptation of application techniques according to the individual needs of each
patient. This can include decisions about the depth of injections, the amount of product to be used,
and the combination with other treatment modalities. This holistic approach tends to provide more
satisfactory and long-lasting results, in addition to increasing patients’ condence in the effectiveness
of the procedures (Farina & Mota, 2023; Miranda & Lopes, 2023).
251
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
In addition to their application in facial treatments, biostimulators are also being investigated
for use in body areas, such as the treatment of accidity in regions such as the abdomen, arms and
thighs. The application of these products to different parts of the body can offer aesthetic results that
go beyond facial rejuvenation, meeting the growing demand for solutions that promote skin rmness
and elasticity in various areas (Papaiordanou et al., 2022; Silva et al., 2022).
Research on biostimulators should also focus on exploring new formulations that may offer
additional benets. For example, combining biostimulators with ingredients that promote hydration
or that have antioxidant properties can enhance the positive effects on the skin. This synergy between
different agents can result in a more effective and comprehensive approach to skin rejuvenation
(Marinho, 2023; Carvalho et al., 2023).
Collaboration between professionals from different areas, such as dermatologists, estheticians,
and nutritionists, can enrich aesthetic practice. This multidisciplinary approach can contribute to the
development of more effective treatment protocols, which consider not only aesthetic aspects, but also
skin health and the patient’s overall well-being (Machado, 2022; Neca et al., 2022).
Transparency in aesthetic practices is essential. Patients should be informed about all aspects
of each treatment, including benets, risks, and realistic expectations. Informed consent is a crucial
component of aesthetic care and should be prioritized by all professionals (Seabra & Silva, 2022;
Troczinski, 2024). Clear communication not only improves patient satisfaction but also helps build a
relationship of trust between the practitioner and the client.
In summary, collagen biostimulators are establishing themselves as a valuable solution for
facial and body rejuvenation. As research and development continue to advance, new techniques
and products must be introduced, expanding the options available to aesthetic professionals. The
integration of science, technology, and a patient-centered approach will be critical to the continued
success of biostimulators in the eld of aesthetic medicine. This evolution will not only provide better
aesthetic results but will also contribute to the emotional well-being and self-esteem of patients,
reecting the importance of aesthetics in contemporary life.
252
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
Safety and the Side Effects Associated with the Use of Collagen Biostimulators in Facial Aesthetic
Procedures
The use of collagen biostimulators in facial aesthetic procedures has become a common
practice in dermatology and aesthetic medicine, due to their ability to stimulate collagen production
and improve the appearance of the skin. Products such as calcium hydroxyapatite (CaHA) and poly-L-
lactic acid (PLLA) act on the deeper layers of the skin, promoting neocollagenesis and providing visible
results over time (Neca et al., 2022; Araújo et al., 2022; Farina et al., 2022). Calcium hydroxyapatite,
for example, not only acts as a ller but also induces a controlled inammatory response that results
in collagen production, contributing to skin revitalization (Silva, 2024; Troczinski, 2024; Oliveira et
al., 2021).
Collagen biostimulators are often used in facial harmonization procedures, with the aim of
restoring lost volume and improving skin texture (Araújo et al., 2022; Veloso, 2023; Seabra & Silva,
2022). The popularity of these treatments stems from their minimally invasive nature, offering a less
risky alternative compared to more invasive surgical procedures (Matos, 2023; Jacintho, 2023).
In addition, the safety of these products is often highlighted, since they are considered
biocompatible and biodegradable, resulting in minimal side effects (Araújo et al., 2022; Jacintho,
2023; Farina et al., 2022). However, it is essential that these procedures are performed by qualied
professionals, as the application technique and the choice of product can signicantly inuence the
results and safety of the treatment (Beserra et al., 2023).
Despite the benets associated with using collagen biostimulators, it is important to consider
the potential side effects. Among the most common adverse effects are inammatory reactions,
bruising, and, in rare cases, more serious complications, such as infections or allergic reactions
(Jacintho, 2023; Veloso, 2023; Beserra et al., 2023). The literature indicates that, although most
adverse reactions are mild and temporary, the occurrence of serious side effects may be related to
253
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
the application technique, the quality of the product used, and the experience of the professional
(Jacintho, 2023; Beserra et al., 2023). Thus, careful selection of the biostimulator and evaluation of the
patient’s conditions are essential to minimize risks.
In addition, individual response to treatment can vary signicantly, inuenced by factors
such as age, skin type, and underlying health conditions (Seabra & Silva, 2022; Veloso, 2023). Studies
show that the effectiveness of collagen biostimulators can be maximized when combined with other
techniques, such as microneedling, which increases product absorption and improves aesthetic results
(Farina & Mota, 2023). This combination of techniques can result in a synergistic effect, providing a
more signicant improvement in sagging and skin texture (Farina & Mota, 2023).
Research on collagen biostimulators continues to evolve, with the development of new
products and techniques that seek to maximize the efcacy and safety of aesthetic treatments. Calcium
hydroxyapatite and poly-L-lactic acid, for example, have been the subject of studies investigating
their mechanisms of action and their applications in different aesthetic contexts (Troczinski, 2024;
Araújo et al., 2022; Oliveira et al., 2021). In addition, the search for more natural alternatives, such as
autologous biostimulators, is gaining prominence, as they offer similar results with potentially lower
risks (Jacintho, 2023; Seabra & Silva, 2022).
The growing acceptance of collagen biostimulators in the aesthetic market has driven the
diversication of available treatments. As more professionals adopt these techniques, continuous
training and updating become essential to ensure the safety and effectiveness of the procedures. It is
essential that health professionals are well informed about the various options for biostimulators, their
indications and contraindications, in addition to developing practical skills to perform appropriate
applications (Matos, 2023; Jacintho, 2023).
Ongoing research on biostimulators is also investigating the effectiveness of product
combinations and techniques. For example, the use of biostimulators together with technologies such
as radiofrequency or ultrasound can enhance the results, offering additional improvements in skin
rmness and elasticity (Farina & Mota, 2023). This integrated approach not only improves treatment
254
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
effectiveness but also provides patients with a more complete and satisfying experience.
The growing concern about the sustainability and safety of aesthetic treatments is generating
a greater demand for products that are effective and also minimize their environmental impact.
Autologous biostimulators, which are obtained from the patient himself, are receiving prominence
for offering a more natural alternative with a lower risk of adverse reactions (Seabra & Silva, 2022;
Jacintho, 2023). This trend reects a change in the perception of patients, who are increasingly looking
for solutions that respect their health and the environment.
Another point to consider is the psychological and emotional impact that aesthetic treatments
can have on patients. The improvement in the appearance of the skin, provided by biostimulators,
often results in an increase in self-esteem and condence, which can have a positive effect on quality
of life. This aspect emphasizes the importance of approaches that consider not only aesthetic outcomes
but also the emotional well-being of patients (Machado, 2022).
Finally, the continuity of research on collagen biostimulators is vital for the advancement of
aesthetic practices. Future studies should focus on evaluating long-term efcacy, safety, and patient
satisfaction. This will ensure that the treatments offered are not only effective but also safe and
sustainable (Veloso, 2023). The combination of technological innovations with the personalization of
treatments will allow aesthetic professionals to meet the growing expectations of patients, solidifying
the role of biostimulators as a preferred option for facial and body rejuvenation.
In summary, collagen biostimulators not only offer an effective solution to sagging and loss
of skin elasticity, but also represent a holistic approach that meets the growing demand for safe and
personalized aesthetic treatments. As research advances and new techniques are developed, these
treatments are expected to continue to evolve, beneting more and more individuals looking to
improve their appearance and well-being.
255
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
DISCUSSION
The discussion about the use of collagen biostimulators in facial aesthetic procedures
shows a growing acceptance and demand for these treatments, supported by a series of studies that
demonstrate their effectiveness in promoting skin elasticity and rmness. The literature review reveals
that biostimulators, such as calcium hydroxyapatite (CaHA) and poly-L-lactic acid (PLLA), stand out
for their ability to stimulate neocollagenesis, resulting in visible improvements in skin appearance
over time (Neca et al., 2022; Farina et al., 2022).
One of the most relevant aspects identied in the literature is the minimally invasive nature
of these procedures, which provides an attractive alternative to more invasive cosmetic surgeries. The
popularity of biostimulators is due not only to their efcacy, but also to their safety, with many studies
favoring the biocompatibility and biodegradability of these products, which result in generally mild
and temporary side effects (Araújo et al., 2022; Jacintho, 2023). This safety is a crucial factor that can
inuence patients’ choice when considering aesthetic treatments.
However, the review also highlights the need for a careful assessment of patients’ conditions
before the application of biostimulators. The response to treatment can vary signicantly between
individuals, being inuenced by factors such as age, skin type, and underlying health conditions
(Seabra & Silva, 2022; Veloso, 2023). The literature suggests that the personalization of treatments is
essential to optimize results and increase patient satisfaction, which implies meticulous planning that
takes into account individual characteristics and expectations regarding the treatment.
In addition, the combination of biostimulators with other aesthetic techniques, such as
microneedling and radiofrequency, has been widely discussed. These combinations can generate
synergistic effects, enhancing aesthetic results and offering a more comprehensive approach to facial
rejuvenation (Farina & Mota, 2023). This integrated treatment strategy is increasingly recognized as
an effective way to maximize the effectiveness of biostimulators, promoting signicant improvements
in sagging and skin texture.
256
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
Another important point highlighted in the literature is the consideration of the emotional
and psychological implications of aesthetic treatments. The improvements in the appearance of the
skin, provided by biostimulators, can have a positive impact on patients’ self-esteem and condence.
This aspect is often overlooked, but it represents an important motivation for many who seek aesthetic
interventions (Machado, 2022). Thus, aesthetic practices must consider not only the physical outcomes,
but also the emotional well-being of patients.
Lastly, the discussion on collagen biostimulators should include the need for ongoing research
to explore new formulations and techniques that can further enhance the efcacy and safety of these
treatments. The search for more natural alternatives, such as autologous biostimulators, is becoming
more common, reecting a trend towards options that minimize risks and offer comparable results
(Seabra & Silva, 2022; Jacintho, 2023).
In summary, the reviewed literature indicates that collagen biostimulators are an effective
and safe solution for facial rejuvenation, but highlights the importance of personalization of treatment,
careful assessment of patients’ conditions, and consideration of the emotional aspects associated with
aesthetic procedures. Continuous development in research and application techniques may result in
signicant improvements in aesthetic practices, meeting the growing demand for treatments that
promote not only beauty, but also the integral well-being of patients.
CONCLUSION
The present study analyzed the use of collagen biostimulators in aesthetic procedures,
highlighting their contributions to skin revitalization and the promotion of satisfactory results in
a minimally invasive way. The proposed objectives, which included understanding the efcacy of
biostimulators and their implications for aesthetic practice, were fully contemplated.
The literature review showed that products such as calcium hydroxyapatite and poly-L-lactic
acid are effective in stimulating collagen production, contributing to signicant improvements in skin
257
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
elasticity and rmness over time. In addition, the safety of these treatments, often attested by studies,
reinforces their acceptance both by professionals in the eld and by patients seeking less invasive
alternatives to surgical procedures.
The problem question, which addressed the efcacy and safety of collagen biostimulators in
comparison with other aesthetic procedures, was also adequately answered. The results indicate that
the individualization of treatments is a crucial factor to optimize results and ensure patient satisfaction.
The analysis of personal factors, such as age, skin type, and health conditions, is essential to minimize
risks and enhance the benets of aesthetic treatments.
Suggestions for future work include conducting more comprehensive comparative studies
between different types of biostimulators and their combinations with other aesthetic techniques. In
addition, investigating the longevity of outcomes from different treatment protocols could provide
valuable information for clinical practice. The exploration of the psychological and emotional impact
of aesthetic interventions on patients’ quality of life also presents itself as a promising area for research,
contributing to a broader understanding of consumers’ needs and expectations.
Finally, the combination of biostimulators with other aesthetic techniques, such as
microneedling and radiofrequency, presents itself as a promising approach, capable of enhancing
results and offering a more comprehensive experience to patients. Continuous research and innovation
in this eld are vital for the advancement of aesthetic practices, allowing professionals to stay up-to-
date with the best options available and the growing expectations of consumers. In this way, collagen
biostimulators are consolidated as an effective and safe choice, reecting a signicant evolution in the
care of aesthetics and skin health.
REFERENCES
ARAÚJO, B.; OLIVEIRA, H.; COSTA, L.; ORSOLIN, P.; ANDRADE, R. The use of semi-permanent
collagen biostimulators in orofacial harmonization. Research Society and Development, v. 11, n. 13,
p. e397111335581, 2022.
258
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
BESERRA, M.; SIQUEIRA, L.; PEIXOTO, F. Implications of the use of collagen biostimulators in
the treatment of the face. Revista Eletrônica Acervo Saúde, v. 23, n. 1, p. e11560, 2023.
BORDALO, S. Use of calcium hydroxyapatite as a dermal ller in patients with HIV-associated facial
lipodystrophies. Contribuciones a Las Ciencias Sociales, v. 17, n. 5, p. e6379, 2024.
TIRED, V. Percutaneous collagen induction in the treatment of acne scars: a technical report.
E-Acadêmica, v. 4, n. 3, p. e0143441, 2023.
CARVALHO, G.; MONTEIRO, L.; WANDERLEY, R.; NUNES, Y.; SILVA, J.; SILVA, E. Oral
collagen supplementation and its effects on the skin. Research Society and Development, v. 12, n. 2,
p. e20312240231, 2023.
DIAS, A.; IGNACIO, P.; LOPES, R.; SOUSA, T.; SANTOS, D. The use of polydioxanone (PDO)
threads for collagen induction: a literature review. Multidisciplinary Scientic Journal Knowledge
Core, p. 99-119, 2022.
DIAZ, L.; SANTOS, V.; CAVALCANTE, A.; HUFFENBAECHER, P.; QUEIROZ, T.; SANTOS, P.;
ROCHA, T. Harmonyca®: a new generation of hybrid injectors. Aesthetic Orofacial Science, v. 3, n.
3, p. 55-62, 2022.
FARINA, T.; MENEGA, T.; MELINE, R. New generation of biostimulators for buttock harmonization.
Scientic Journal of Aesthetics and Cosmetology, v. 2, n. 1, p. E0582022, 2022.
FARINA, T.; MOTA, L. Therapy combining microneedling and collagen biostimulator in the treatment
of skin sagging in a bariatric patient – a qualitative case study. Scientic Journal of Aesthetics and
Cosmetology, v. 3, n. 1, 2023.
GARCIA, F. Use of collagen biostimulators in orofacial harmonization: a literature review. Journal of
Multidisciplinary Dentistry, v. 13, n. 3, p. 43-50, 2024.
JACINTHO, G. Autologous biostimulators: good results with more safety. Recima21 - Multidisciplinary
Scientic Journal, v. 4, n. 10, p. e4104228, 2023.
259
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
LIMA, N.; SOARES, M. Use of collagen biostimulators in orofacial harmonization. Clinical and
Laboratorial Research in Dentistry, 2020.
LUÍZ, L. Calcium hydroxyapatite in orofacial harmonization: a narrative review of the literature.
Research Society and Development, v. 12, n. 7, p. e2712742498, 2023.
MACHADO, S. Benets of hyaluronic acid in orofacial harmonization. Research Society and
Development, v. 12, n. 8, p. e6912842894, 2023.
MARINHO, V. PDO threads in orofacial harmonization: a narrative review of the literature. Research
Society and Development, v. 12, n. 6, p. e9212642113, 2023.
MATOS, E. Collagen biostimulators in the treatment of skin aging and the role of the pharmacist.
Research Society and Development, v. 12, n. 14, p. e05121444423, 2023.
MIYASHIRO, R.; COSTA, D.; DEUSCHLE, V. Collagen supplementation for skin aging: a review.
Biomotriz, v. 16, n. 1, 2022.
MOURA, M. Use of calcium hydroxyapatite and hyaluronic acid (harmonycatm) as a facial ller.
Brazilian Journal of Implantology and Health Sciences, v. 5, n. 5, p. 3707-3725, 2023.
NECA, C.; GONDIM, A.; ROCHA, C.; SILVA, C.; SILVA, F. The use of collagen biostimulators
based on calcium hydroxyapatite. E-Acadêmica, v. 3, n. 2, p. e7332237, 2022.
NOGUEIRA, I.; SILVA, N. Applicability of collagen biostimulators (poly-l-lactic acid and calcium
hydroxyapatite) in dermal llers in off-face areas of the body. Research Society and Development, v.
11, n. 8, p. e47411831181, 2022.
OLIVEIRA, C.; ALMEIDA, T.; MARTINS, L.; SORPRESO, L.; FINCK, N. Calcium hydroxyapatite:
a review for efcacy, safety, and imaging when used as a ller and as a biostimulator. Research
Society and Development, v. 10, n. 14, p. e05101421689, 2021.
PAPAIORDANOU, F.; DE-OLIVEIRA, G.; HEXSEL, D.; VATTIMO, A. Collagen and skin: from
structure to evidence of oral supplementation. Surgical & Cosmetic Dermatology, Vol. 14, 2022.
260
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
PIRES, Y.; RIBEIRO, P. Orofacial harmonization and the use of hyaluronic acid and botulinic toxin:
the power to restore self-esteem. Id on Line Journal of Psychology, v. 15, n. 56, p. 252-260, 2021.
POGERE, A.; PERES, G.; SILVA, J.; STIMAMIGLIO, M.; SETTI, J.; BERTI, L. Data collection
for systematic review for in vitro biological response of magnesium-doped calcium phosphate-based
bioceramics. Brazilian Journal of Development, v. 7, n. 10, p. 100317-100330, 2021.
RODRIGUES, C.; PACHECO, C.; NAVES, M.; SOUZA, D.; PACHECO, R. Orofacial harmonization
in the reestablishment of self-esteem: a case report. Aesthetic Orofacial Science, v. 3, n. 1, p. 19-28,
2022.
ZARAGOZA, A. Collagen biostimulators (polylactic acid, calcium hydroxyapatite, polycaprolactone
and polydioxanone). Recima21 - Multidisciplinary Scientic Journal, v. 4, n. 1, 2023.
SEABRA, A.; SILVA, D. Collagen biostimulator in facial harmonization: a literature review. Research
Society and Development, v. 11, n. 14, p. e426111435713, 2022.
SANTOS, F.; BRITO, J.; BRILHANTE, J.; QUEIROZ, L.; PENA, S. Association of therapies for
facial rejuvenation - case report. Journal of Multidisciplinary Dentistry, v. 11, n. 1, p. 249-56, 2023.
SILVA, A. Efcacy of calcium hydroxyapatite in tissue sagging of mature skin. Revista Contemporânea,
v. 4, n. 4, p. e4132, 2024.
SILVA, S.; SOUZA, M.; SOUZA, D. Facial use of pdo threads and complications after aesthetic
treatment. Aesthetic Orofacial Science, v. 3, n. 3, p. 63-71, 2022.
TROCZINSKI, A. The use of calcium hydroxyapatite as a collagen biostimulator in aesthetic
biomedicine: a literature review. Brazilian Journal of Implantology and Health Sciences, v. 6, n. 5, p.
1289-1312, 2024.
VASCONCELOS, R.; DURS, L.; DUARTE, A. Reconstruction of facial hemiatrophy with calcium
hydroxyapatite: report of a safe and minimally invasive technique. Surgical & Cosmetic Dermatology,
Vol. 14, 2022.
VELOSO, L. The use of injectable collagen biostimulators to control signs of facial aging. Recima21
261
ISSN: 2763-5724 / Vol. 05 - n 04 - ano 2025
- Multidisciplinary Scientic Journal, v. 4, n. 6, p. e463296, 2023.
